Thermo Scientific™ DAPI (4',6-diamidino-2-phenylindole, dihydrochloride)

Thermo Scientific™ DAPI (4',6-diamidino-2-phenylindole, dihydrochloride)

Thermo Scientific Pierce DAPI Nuclear Counterstains are high-purity forms of diamidino-2-phenylindole dye for fixed-cell, fluorescent staining of DNA content and nuclei for cellular imaging techniques. DAPI (diamidino-2-phenylindole) is a blue fluorescent probe that fluoresces brightly upon selectively binding to the minor groove of double stranded DNA, where its fluorescence is approximately 20-fold greater than in the nonbound state. Its selectivity for DNA and high cell permeability allows efficient staining of nuclei with little background from the cytoplasm. DAPI is a classic nuclear counterstain for immunofluorescence microscopy, as well as an important component of high-content screening methods requiring cell-based quantitation of DNA conten

Features

• DAPI dye—diamidino-2-phenylindole is a blue fluorescent stain specific for DNA
• Convenient—choose powder or easy-to-use DAPI dye solution (1 mg/mL)
• Cellular imaging—cell-permeable dye is effective for fixed-cell staining and quantitation of DNA content
• Counterstain—ideal for use alongside detection of specific targets with fluorescent antibodies for fluorescence microscopy or high-content screening (HCS)
• SubCellular Localization: Nucleus
• For Use With (Equipment): Fluorescence Microscope, High Content Instrument