Thermo Scientific™ DSP (dithiobis(succinimidyl propionate)), Lomant's Reagent, 50 mg
On demandThermo Scientific™ DSP (dithiobis(succinimidyl propionate)), Lomant's Reagent, 50 mg
Thermo Scientific Pierce DSP (Lomant's Reagent) is a water-insoluble, homo-bifunctional N-hydroxysuccimide ester (NHS ester) crosslinker that is thiol-cleavable, primary amine-reactive, and useful for many applications. DSP contains an amine-reactive NHS ester at each end of an 8-carbon spacer arm. NHS esters react with primary amines at pH 7–9 to form stable amide bonds and releasing N-hydroxy-succinimide. Proteins, including antibodies, generally have several primary amines in the side chain of lysine (K) residues and the N-terminus of each polypeptide that are available as targets for NHS ester crosslinking reagents. DSP is non-sulfonated and insoluble in water, so it must first be dissolved in an organic solvent and then added to the aqueous reaction mixture. Because DSP does not possess a charged group, it is lipophilic and membrane-permeable and so useful for intracellular and intramembrane conjugation. A sulfonated analog of DSP (DTTSP) is water soluble. DSS, the non-cleavable analog of the DSP crosslinker is also available for applications that require a stable spacer arm that cannot be cleaved in the presence of reducing agents.
Features
- Reactive groups: NHS ester (both ends)
- Reactive towards: amino groups (primary amines)
- Amine-reactive NHS ester reacts rapidly with any primary amine-containing molecule
- Disulfide bond in the spacer arm is readily cleaved by 10-50 mM DTT or TCEP at pH 8.5
- Spacer arm also cleavable with reducing SDS-PAGE sample loading buffer
- Cleavable crosslinker allows separation of crosslinked products
- Water-insoluble (dissolve first in DMF or DMSO); compare to DTSSP (Sulfo-DSP)
- Membrane-permeable, allowing for intracellular crosslinking
There are no specifications
There are no report
You May Also Like
![](https://laboshop.ae/public/storage/files/5ec73d02-825c-484d-9692-ab9943ecc558.jpg)
Thermo Scientific™ EZ-Link™ Micro Sulfo-NHS-LC-Biotinylation Kit
$ On demand
On demand
![](https://laboshop.ae/public/storage/files/faf776a0-5e5c-4a5a-86b6-dc85231c9f01.jpg)
Invitrogen™ Alexa Fluor™ 532 Carboxylic Acid, triethylammonium salt
$ On demand
On demand
![](https://laboshop.ae/public/storage/files/98844340-93b0-4982-9e1b-267922904287.jpg)
Invitrogen™ Zenon™ Mouse IgG1 Labeling Kit, R-PE (R-Phycoerythrin)
$ On demand
On demand
![](https://laboshop.ae/public/storage/files/gPWBJ3An8zR62005g7b5zZHaFStXN1L4d5buZNAx.jpg)
Invitrogen™ Alexa Fluor™ 594 NHS Ester (Succinimidyl Ester), 1 mg
$ On demand