Thermo Scientific™ EZ-Link™ Micro NHS-PEG4-Biotinylation Kit
On demand
Order number :
21955
Thermo Scientific™ EZ-Link™ Micro NHS-PEG4-Biotinylation Kit
The Thermo Scientific EZ-Link NHS-PEG4 Biotinylation Kit contains EZ-Link NHS-PEG4-Biotin and accessory reagents required for 8 0.05–0.2 mg protein labeling reactions. EZ-Link NHS-PEG4-Biotin is a pegylated, water-soluble reagent for simple and efficient biotin labeling of antibodies, proteins and other primary amine-containing macromolecules.
Features
- Protein labeling—biotinylate antibodies or other proteins for detection or purification using streptavidin probes or resins
- Amine-reactive—reacts with primary amines (-NH2), such as the side-chain of lysines (K) or the amino-termini of polypeptides
- Pegylated – spacer arm contains a hydrophilic, 4-unit, polyethylene glycol (PEG) group
- Enhances solubility – pegylation imparts water solubility to the biotinylated molecule, helping to prevent aggregation of biotinylated antibodies stored in solution
- Irreversible—forms permanent amide bonds; spacer arm cannot be cleaved
- Long reach – spacer arm (total length added to target) is 29 angstroms; this reduces steric hindrance when binding to avidin molecules
Contents
Sufficient For: 8 labeling reactions, each with 0.05–0.2 mg of antibody
- EZ-Link NHS-PEG4-Biotin: 8 x 2 mg
- PBS Pack (makes 500 mL): 1 pack
- Zeba Spin Desalting Columns (7K MWCO): 2 mL, 10 columns
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