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Thermo Scientific™ EZ-Link™ Micro NHS-PEG4-Biotinylation Kit

On demand
Order number : 21955
(Ex-work price)
$ On demand

Thermo Scientific™ EZ-Link™ Micro NHS-PEG4-Biotinylation Kit


The Thermo Scientific EZ-Link NHS-PEG4 Biotinylation Kit contains EZ-Link NHS-PEG4-Biotin and accessory reagents required for 8 0.05–0.2 mg protein labeling reactions. EZ-Link NHS-PEG4-Biotin is a pegylated, water-soluble reagent for simple and efficient biotin labeling of antibodies, proteins and other primary amine-containing macromolecules.


Features

  • Protein labeling—biotinylate antibodies or other proteins for detection or purification using streptavidin probes or resins
  • Amine-reactive—reacts with primary amines (-NH2), such as the side-chain of lysines (K) or the amino-termini of polypeptides
  • Pegylated – spacer arm contains a hydrophilic, 4-unit, polyethylene glycol (PEG) group
  • Enhances solubility – pegylation imparts water solubility to the biotinylated molecule, helping to prevent aggregation of biotinylated antibodies stored in solution
  • Irreversible—forms permanent amide bonds; spacer arm cannot be cleaved
  • Long reach – spacer arm (total length added to target) is 29 angstroms; this reduces steric hindrance when binding to avidin molecules


Contents

Sufficient For: 8 labeling reactions, each with 0.05–0.2 mg of antibody

  • EZ-Link NHS-PEG4-Biotin: 8 x 2 mg
  • PBS Pack (makes 500 mL): 1 pack
  • Zeba Spin Desalting Columns (7K MWCO): 2 mL, 10 columns


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