Thermo Scientific™ Pierce™ SM(PEG)12, No-Weigh™ Format, 10 x 1 mg

Thermo Scientific™ Pierce™ SM(PEG)12, No-Weigh™ Format, 10 x 1 mg

Thermo Scientific Pierce SM(PEG)12 is a hetero-bifunctional crosslinker with N-hydroxysuccinimide (NHS) ester and maleimide groups that allow covalent conjugation of amine- and sulfhydryl-containing molecules. Crosslinkers having polyethylene glycol (PEG) spacers are convenient alternatives to reagents with purely hydrocarbon spacer arms. PEG spacers improve water solubility of reagent and conjugate, reduce the potential for aggregation of the conjugate, and increase flexibility of the crosslink, resulting in reduced immunogenic response to the spacer itself. In contrast to typical PEG reagents that contain heterogeneous mixtures of different PEG chain lengths, Pierce PEGylation reagents are homogeneous compounds of defined molecular weight and spacer arm length, providing greater precision in optimization and characterization of crosslinking applications.

Thermo Scientific No-Weigh products are specialty reagents provided in a pre-aliquoted format. The pre-weighed packaging prevents the loss of reagent reactivity and contamination over time by eliminating the repetitive opening and closing of the vial. The format enables use of a fresh vial of reagent each time, eliminating the hassle of weighing small amounts of reagents and reducing concerns over reagent stability.

Features

• Reactive groups: NHS ester and maleimide
• Reactive toward: amino and sulfhydryl groups
• Irreversibly crosslink proteins or peptides by flexible PEG spacer arms
• Polyethylene glycol spacer arms help maintain conjugate solubility
• Pure compound with defined structure and molecular weight, ensuring reproducible protein-modification effects
• PEG spacer provides unique advantages, including increased stability, reduced tendency toward aggregation and reduced immunogenicity
• Ideal for performing controlled, two-step protein conjugations (see Sulfo-SMCC)