Thermo Scientific™ High-Select™ TiO2 Phosphopeptide Enrichment Kit

Thermo Scientific™ High-Select™ TiO2 Phosphopeptide Enrichment Kit

The Thermo Scientific™ High-Select™ TiO2 Phosphopeptide Enrichment Kit enables efficient enrichment of phosphorylated peptides from complex and fractionated protein digests for analysis by mass spectrometry (MS). 

The complete kit includes 24 titanium dioxide (TiO2) spin tips with optimized buffers to facilitate preparation of enriched phosphopeptides. Each column can enrich phosphopeptides from 0.5 to 3 mg of total protein digest as starting sample. The optimized buffers and simplified procedure eliminate the need for pyrrolidine in the elution buffer and subsequent clean-up using graphite spin columns. Use of this kit results in enrichment of up to 150 µg of phosphopeptides with greater than 90% selectivity.

For use with: Microcentrifuge

Features

• Convenient—pre-formulated buffers for ease of use and spin-tip format to enable parallel processing of multiple samples
• High binding capacity—each column enriches up to 150 μg of phosphopeptides
• High specificity—recovers phosphopeptides with > 90% selectivity
• Complementary—TiO2 enriches a unique set of phosphopeptides that complements the High-Select™ Fe-NTA IMAC Phosphopeptide Enrichment Kit

Contents

• TiO2 Spin Tips (24)
• Centrifuge Column Adadptors (24)
• Binding/Equilibration Buffer (7 mL)
• Wash Buffer (1.8 mL)
• Phosphopeptide Elution Buffer (7 mL)