Thermo Scientific™ SILAC Protein Quantitation Kit (LysC), DMEM:F-12
On demand
Order number :
A33970
Thermo Scientific™ SILAC Protein Quantitation Kit (LysC), DMEM:F-12
The Thermo Scientific SILAC Protein Quantitation Kit - DMEM:F12 is used for the specific analysis of protein expression by mass spectrometry using stable isotope labeling with amino acids in cell culture (SILAC).
Features
- Efficient—up to 100% label incorporation into proteins of living cells
- Reproducible—minimizes intra-experimental variability caused by differential sample preparation
- Flexible—media deficient in both L-lysine and L-arginine, allowing for more complete proteome coverage through dual amino acid isotope labeling
- Compatible—label proteins expressed in a wide variety of mammalian cell lines adapted to grow in DMEM:F12 medium, including HeLa, 293T, COS7, U2OS, A549, A431, HepG2, NIH 3T3, Jurkat and others
- High-quality supplements—heavy amino acids with >99% isotope purity; dialyzed FBS tested to help ensure that it is sterile, endotoxin-free, and cell culture compatible
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