Thermo Scientific™ SPDP (succinimidyl 3-(2-pyridyldithio)propionate)
On demand
Order number :
21857
Thermo Scientific™ SPDP (succinimidyl 3-(2-pyridyldithio)propionate)
Thermo Scientific Pierce SPDP is a short-chain crosslinker for amine-to-sulfhydryl conjugation via NHS-ester and pyridyldithiol reactive groups that form cleavable (reducible) disulfide bonds with cysteine sulfhydryls.
Features
- Reactive groups: NHS ester and pyridyldithiol
- Reactive towards: amino and sulfhydryl groups
- Releases a detectable by-product when reacted with a free sulfhydryl group; by measuring the release of pyridine-2-thione at 343 nm, the reaction can be easily followed
- Disulfide bond in the spacer arm is readily cleaved by 10-50 mM DTT or TCEP at pH 8.5
- Spacer arm is also easily cleaved using reducing SDS-PAGE sample loading buffer
- Cleavable crosslinker allows separation of crosslinked products
- Water-insoluble (dissolve first in DMF or DMSO)
- SPDP crosslinker is membrane permeable, so crosslinking can be done inside the cells
- Compare to other varieties of SPDP-type reagents, including pegylated forms
There are no specifications
There are no report
You May Also Like
Invitrogen™ ARP (N-(aminooxyacetyl)-N'-(D-Biotinoyl) hydrazine, Trifluoroacetic Acid Salt)
(Ex-work price)
$ On demand
$ On demand
On demand
Thermo Scientific™ Pierce™ Cypridina Luciferase Glow Assay Kit, 1000 Reactions
(Ex-work price)
$ On demand
$ On demand
On demand
Thermo Scientific™ Sulfo-SBED Biotin Label Transfer Reagent, 10 mg
(Ex-work price)
$ On demand
$ On demand
On demand
Thermo Scientific™ Sulfo-SDAD (Sulfo-NHS-SS-Diazirine) (sulfosuccinimidyl 2-((4,4'-azipentanamido)ethyl)-1,3'-dithiopropionate)
(Ex-work price)
$ On demand
$ On demand
On demand
Thermo Scientific™ CA(PEG)12 Carboxy-PEG-Amine Compound, 100 mg
(Ex-work price)
$ On demand
$ On demand