Thermo Scientific™ Pierce™ Crosslink Magnetic IP/Co-IP Kit

Thermo Scientific™ Pierce™ Crosslink Magnetic IP/Co-IP Kit

The Thermo Scientific Pierce Crosslink Magnetic IP/Co-IP Kit uses crosslinking chemistry to covalently immobilize IP antibodies onto premium-quality Protein A/G Magnetic Beads for effective immunoprecipitation and co-immunoprecipitation.

Because this magnetic IP procedure involves covalent attachment of the specific antibody used for immunoprecipitation, target proteins or co-IP protein complexes can be eluted and analyzed without antibody fragments because the antibody remains affixed to the beads. The kit contains an optimized protocol and all buffers and reagents necessary to accomplish high-yield IP or co-IP with antibodies that bind to Protein A/G and using either manual or automated magnetic separation tools.

Features

• Antibody-free IP—antibody is irreversibly attached to the magnetic beads resulting in negligible co-elution of intact antibody or heavy and light chains with the purified antigen
• Convenient—the IP/co-IP kit contains magnetic beads and all essential buffers for optimal immunoprecipitation
• Fast—complete crosslinking and immunoprecipitation in less than 3 hours
• Efficient—immunoprecipitate with half the recommended volume of magnetic particles compared to other magnetic beads
• Compatible—magnetic beads coupled with Protein A/G recombinant protein ensures compatibility with most primary antibodies, whether from mouse or rabbit
• Scalable—use only the amount of antibody needed for a single IP experiment or prepare a larger quantity of antibody-crosslinked magnetic beads for multiple experiments

Contents

• Pierce Protein A/G Magnetic Beads: 1 mL
• IP Lysis/Wash Buffer: 2 x 50 mL
• Coupling Buffer (20X): 25 mL
• DSS Crosslinker: 8 x 2 mg
• Lane Marker Sample Buffer (5X): 5 mL
• Elution Buffer: 25 mL
• Neutralization Buffer: 1 mL
• Lane Marker Sample Buffer (5X): 5 mL