Thermo Scientific™ Compat-Able™ BCA Protein Assay Kit

Thermo Scientific™ Compat-Able™ BCA Protein Assay Kit

The Compat-Able BCA Protein Assay Kit eliminates interfering substances from protein assay samples to make them compatible with BCA and Bradford assays.

The Compat-Able reagents included in this protein assay kit remove salts, detergents, reducing agents, and other substances from protein samples to eliminate interference with protein assays. The protocol selectively precipitates all of the protein, thereby allowing the non-protein components to be easily decanted and removed. The purified, precipitated protein is re-dissolved in water, making it ready for accurate determination of the protein concentration by standard protein assay methods. The procedure is accomplished in less than 10 minutes with just a few simple steps. The Compat-Able reagents are also available individually.

For use with equipment: Spectrophotometer, Microplate Reader

Features

• Eliminate incompatibilities—removes nearly any substance that interferes with colorimetric protein assays: salts, detergents, denaturants, reducing agents, and even IEF sample buffer
• Simple and fast—prepare samples in less than 10 minutes with four simple steps
• Convenient—reagent set is easier to use than homemade TCA or acetone precipitation reagents, thereby producing more consistent results
• Scalable—microcentrifuge method is easily scaled to larger centrifuge tube volumes for special needs
• Validated—tested and offered together with BCA and Coomassie Plus Protein Assay Kits; protocol integrates control and normalization procedures

Contents

• Compat-Able Reagent 1, 250 mL
• Compat-Able Reagent 2, 250 mL
• BCA Reagent A, 500 mL
• BCA Reagent B, 25 mL
• Albumin Standard Ampules, 2 mg/mL, 10 x 1 mL