Thermo Scientific™ Pierce™ Quantitative Colorimetric Peptide Assay

Thermo Scientific™ Pierce™ Quantitative Colorimetric Peptide Assay

The Thermo Scientific™ Pierce™ Quantitative Colorimetric Peptide Assay is an easy-to-use colorimetric microplate assay designed specifically to improve the sensitivity and reproducibility of quantitation of peptide mixtures.

The Pierce Quantitative Colorimetric Peptide Assay provides modified BCA reagents for the reduction of Cu+2 to Cu+1 and a proprietary chelator that have been optimized for the quantitation of peptide mixtures. In this reaction, the copper is first reduced by the amide backbone of peptides under alkaline conditions (Biuret reaction). The proprietary chelator then couples with the reduced copper to form a bright red complex with absorbance of 480 nm. The signal produced from this reaction is 3-4 fold more sensitive than the Micro-BCA Protein Assay for peptide analysis.

For use with: Microplate Reader

Features

• Sensitive—accurately detect as little as 25 µg/mL of peptide mixture
• Robust—assay performance rigorously tested using peptide digest mixtures
• Robust peptide digest standard—kit includes a validated peptide digest standard for improved reproducibility of quantitation
• Compatible—works with many reagents, including mass spectrometry sample preparation reagents and TMT™-labeled peptides
• Convenient—easy mix-and read-format and stable colorimetric signal that may be read in as little as 15 minutes

Contents

• Colorimettric Assay Reagent A: 50 mL
• Colorimetric Assay Reagent B: 2 x 25 mL
• Colorimetric Assay Reagent C: 2 mL
• Peptide Digest Assay Standard: 1.5 mL