Thermo Scientific™ Pierce™ Quantitative Fluorometric Peptide Assay

Thermo Scientific™ Pierce™ Quantitative Fluorometric Peptide Assay

The Thermo Scientific™ Pierce™ Quantitative Fluorescent Peptide Assay is a sensitive, mix-and-read fluorescent microplate assay for the quantitative measurement of peptides and peptide mixtures.

The Pierce Quantitative Fluorescent Peptide Assay reagents include peptide assay buffer, fluorescent peptide labeling reagent, and a peptide digest assay standard for the quantitative measurement of peptide concentrations. In this assay, peptides are specifically labeled at the amino-terminus using an amine-reactive fluorescent reagent, and the fluorescently labeled peptides are detected using ex390/em475.

For use with: Microplate Reader

Features

• Sensitive—accurately detect as little as 5.0 µg/mL of single peptides or peptide mixtures
• Robust— assay performance rigorously tested using both peptides and peptide digest mixtures
• Robust peptide digest standard—kit includes a validated peptide digest standard for improved reproducibility of quantitation
• Compatible—works with many reagents, including those used in mass spectrometry sample preparation
• Convenient—easy mix-and-read format and stable fluorescent signal that may be read in as little as 5 minutes up to several hours

Contents

• Fluorometric Peptide Assay Reagent: 4 x 2.5 mL
• Fluorometric Peptide Assay Buffer: 50 mL
• Peptide Digest Assay Standard: 1.5 mL