Invitrogen™ InsectSelect™ Glow Kit with Sf9 Cells

Invitrogen™ InsectSelect™ Glow Kit with Sf9 Cells

The InsectSelect™ Glow Kit features the pIZT/V5-His vector for high-level expression of your
gene of interest. This vector has the following features:
In addition, pIZT/V5-His expresses a fusion of the green fluorescent protein and the Zeocin™ resistance
protein (Zeo-GFP). The Zeo-GFP fusion protein permits rapid selection of stably transfected cell lines
with Zeocin™ and confers a fluorescent phenotype that simplifies identification of transfected cells.

Features

• The OpIE2 promoter for constitutive expression
• The Zeocin™ resistance gene for rapid selection of stably transfected cell lines
• C-terminal V5 epitope and polyhistidine (6xHis) sequence for detection with Invitrogen's Anti-V5 Antibody and rapid purification with nickel-chelating resin
  
  

Contents

• pIZT/V5-His: 40 μL
• pIZT/V5-His/CAT: 40 μL
• OpIE2 Forward Sequencing Primer: 2 μg
• OpIE2 Reverse Sequencing Primer: 2 μg
• OpIE2 Forward Primer: 329 pMoles
• OpIE2 Reverse Primer: 250 pMoles