Thermo Scientific™ SuperSignal™ Western Blot Substrate Trial Pack, Atto & Pico PLUS

Thermo Scientific™ SuperSignal™ Western Blot Substrate Trial Pack, Atto & Pico PLUS

This substrate trial pack contains sample sizes of two substrates: SuperSignal West Atto Ultimate Sensitivity Chemiluminescent Substrate and SuperSignal West Pico PLUS Chemiluminescent Substrate to enable trial use of the most recommended substrates for routine and high-sensitivity western blotting.

Thermo Scientific SuperSignal West Atto Ultimate Sensitivity Chemiluminescent Substrate is an ultra-sensitive enhanced chemiluminescent (ECL) substrate for high attogram protein level detection by western blot analysis with horseradish peroxidase (HRP) enzyme. It is designed for detection of very low-abundant targets or the maximum levels of sensitivity required when using precious samples or costly antibodies.

Features

• Sensitivity: low-femtogram to high-attogram
• Stability: eight hours for working solution, one year for kit at 4°C
• Compatibility: nitrocellulose and PVDF membranes
• Signal duration: up to four hours
• Recommended primary antibody concentration: 1:1,000–1:5,000 dilution (0.2–1.0 µg/mL)
• Recommended secondary antibody concentration: 1:100,000–1:250,000 dilution (4–10 ng/mL)

Contents

•  SuperSignal West Pico PLUS reagent: 20 mL
• SuperSignal West Atto reagent: 20 mL