Thermo Scientific™ Biotin Chromogenic Detection Kit, 30 Reactions

Thermo Scientific™ Biotin Chromogenic Detection Kit, 30 Reactions

Thermo Scientific Biotin Chromogenic Detection Kit is a convenient tool for the chromogenic detection of biotinylated nucleic acid probes. The kit is optimized to reproducibly provide high sensitivity with a low background in applications, such as Southern, Northern, dot and slot blotting, and screening of viral plaques and bacterial colonies. Biotinylation of DNA/RNA probes is widely used as a safe and convenient alternative to radioactive labeling. Biotin can be incorporated into nucleic acids using various enzymatic or non-enzymatic methods, including the Biotin DecaLabel DNA Labeling Kit.
Biotinylated probes are detected with streptavidin coupled to alkaline phosphatase (AP). Streptavidin-AP conjugates bind specifically and irreversibly to the biotin-labeled probes. The probes are then visualized using a chromogenic substrate for alkaline phosphatase BCIP/NBT, which produces a blue-purple precipitate. Visualization does not require X-ray film or other specific equipment.

Features

• High sensitivity of detection—0.03 pg of homologous DNA in dot blot hybridization with biotin-labeled probe
• Low background due to optimized washing and blocking procedures
• Non-radioactive detection visualization does not require X-ray film or other specific equipment
• Fast and convenient—ready-to-use components
  
  

Contents

• Streptavidin-AP Conjugate: 120 μL
• 50X BCIP/NBT Solution: 6 × 2 mL
• Blocking Reagent (powder): 15 g
• 10X Blocking/Washing Buffer: 3 × 200 mL
• 10X Detection Buffer: 90 mL