Invitrogen™ eBioscience™ Cell Proliferation Dye eFluor™ 450, 4 x 500 µg
On demandInvitrogen™ eBioscience™ Cell Proliferation Dye eFluor™ 450, 4 x 500 µg
Cell
Proliferation Dye eFluor™ 450 is a violet fluorescent dye that can be
used to monitor individual cell divisions. This fluorescent dye binds to
any cellular proteins containing primary amines, and as cells divide,
the dye is distributed equally between daughter cells that can be
measured as successive halving of the fluorescence intensity of the dye.
Up to 7 generations may be visualized. Cells labeled with Cell
Proliferation Dye eFluor™ 450 may be fixed and permeabilized for
analysis of intracellular targets using standard formaldehyde-containing
fixatives and saponin-based permeabilization buffers, such as the Foxp3
Transcription Factor Staining Buffer Set or the IC
Fixation Buffer and Permeabilization Buffer (10X).
Cell
Proliferation Dye eFluor™ 450 has a peak excitation of 409 nm and can
be detected using a 450/50 band pass filter (equivalent to eFluor™ 450
or Pacific Blue™), making it compatible with applications that utilize
GFP.
Cell
Proliferation Dye eFluor™ 450 is supplied as a lyophilized powder. Each
vial may be reconstituted to a stock concentration of 10 mM with 165 µL
of anhydrous DMSO; once reconstituted it should be used within 6 months
and protected from light and stored at -20°C with dessicant. Avoid
freeze-thawing.
There are no specifications
There are no report
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