Invitrogen™ pYES2.1 TOPO™ TA Yeast Expression Kit
On demand
Order number :
K415001
Invitrogen™ pYES2.1 TOPO™ TA Yeast Expression Kit
The pYES2.1 TOPO TA Expression Kit offers direct cloning of a Taq-amplified PCR product into a Saccharomyces cerevisiae expression vector. The kit uses the linearized, topoisomerase I-activated pYES2.1/V5-His-TOPO ¤ vector for 5-minute cloning on your bench top and produces >85% recombinants.
Features
- 2µ origin of replication for high-copy maintenance gene for auxotrophic selection on economical minimal media
- C-terminal V5 epitope and polyhistidine (6xHis) tags for efficient detection and purification
Contents
- The pYES2.1 TOPO™ TA box contains all of the reagents necessary for ligation, including 200 ng of topoisomerase I-activated pYES2.1/V5-His-TOPO™ vector
- sterile water
- dNTPs
- 10X PCR buffer
- salt solution
- control template and primers
- GAL1 Forward and V5 C-term Reverse Primers for sequencing or PCR screening
- an expression control plasmid
- The One Shot™ Box contains all of the reagents necessary for transformation, including twenty-one 50 µl aliquots of chemically competent TOP10F´ E. coli
- S.O.C. medium
- supercoiled plasmid control pUC19.
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