Invitrogen™ BsdCassette™ Vector (pCMV/Bsd)
On demand
Brand origin :
United States
Expiry time :
On demand
Delivery time :
1 Week/s
Delivery cost :
On demand
Unit size :
20 µg
Order number :
V51020
Invitrogen™ BsdCassette™ Vector (pCMV/Bsd)
Blasticidin is a potent selection agent that allows selection of stable mammalian cell lines in as little as one week. The BsdCassette™ vectors simplify the construction of custom vectors that use Blasticidin resistance as a selectable marker.
Each BsdCassette™ vector carries the synthetic EM-7 promoter for expression of the bsd gene in E. coli. This eliminates the need to add a second antibiotic resistance gene for selection in E. coli. BsdCassette™ vectors provide a eukaryotic promoter for expressionin yeast (pTEF1/Bsd) or mammalian cells (pCMV/Bsd, pEF1/Bsd, pUB/Bsd) for construction of expression vectors with Blasticidin selection for a wide range of host cell types.
Features
- A strong host promoter to drive expression of the Blasticidin resistance gene (bsd)
- Two large polylinkers flanking the promoter and bsd gene to facilitate the construction of Blasticidin resistant vectors
Contents
- This manual is supplied with the BsdCassette™ vector (pCMV/Bsd). The vector (20 μg) is supplied at 0.5 μg/μL in 10 mM Tris-HCl, 1 mM EDTA, pH 8.0 in a total volume of 40 μL
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