Thermo Scientific™ DH5α Competent Cells

Thermo Scientific™ DH5α Competent Cells

Thermo Scientific DH5α Competent Cells are high efficiency, chemically competent E. coli cells ideal for construction of gene banks or generation of cDNA libraries using plasmid-derived vectors. The φ80dlacZΔM15 marker provides α-complementation of the β-galactosidase gene from pUC or similar vectors to allow blue/white colony screening on bacterial agar plates containing Bluo-Gal or X-Gal. RecA1 and endA1 mutations in DH5α cells improve insert stability and quality of the extracted plasmid DNA.

Features

• High transformation efficiency: >1x109 cfu/µg pUC19 DNA
• Suitable for routine and high-throughput cloning applications
• Genetic markers that allow for blue/white colony screening
• Convenient two reactions per vial packaging
• S.O.C. Outgrowth medium included

Contents

• 10 x DH5α Competent Cells (100 µL)
• pUC19 DNA (50 µL) (10 pg/µL)
• S.O.C. Medium (5 mL)