Invitrogen™ TrueTag™ Donor DNA Kit, BFP
On demand
Order number :
A53806
Invitrogen™ TrueTag™ Donor DNA Kit, BFP
The Invitrogen TrueTag Donor DNA Kit, BFP, provides a rapid solution to drive high-efficiency gene tagging (up to 100% edited cells) with transfection-ready donor DNA for genome editing experiments.
Features
Benefits of the TrueTag Donor DNA Kit include:
- One-step PCR amplification to generate donor templates—no cloning required
- Generate transfection-ready DNA donors in hours instead of days
- Enrich up to 100% BFP-tagged cells
The TrueTag Donor DNA Kit, BFP, provides everything you need to produce a high-quality DNA donor to tag your gene with a selection marker and BFP. Simply provide two locus-specific primers to add homology arms, PCR-amplify, and purify for a transfection-ready donor in hours, not days. Each kit is designed to enable you to:
- Tag the carboxy- (C-) or amino- (N-) terminus of your gene of interest
- Choose from puromycin or blasticidin selection markers—whatever best suits your cell model
- Easily remove antibiotic resistance markers with Cre/Lox following cell enrichment
Included in the kit
- Four linear donor templates: N-terminus/puromycin resistance, N-terminus/blasticidin resistance, C-terminus/puromycin resistance, C-terminus/blasticidin resistance
- Phusion Flash High-Fidelity PCR Master Mix
- PCR cleanup columns and buffers
- Positive control primers to tag human beta-actin (ACTB)
Contents
- TrueTag Assembly Kit
- TrueTag Cleanup Kit
There are no specifications
There are no report
You May Also Like
![](https://laboshop.ae/public/storage/files/758541a5-49e1-4bf7-a24f-5fc98c871a50.jpg)
Thermo Scientific™ pT7CFE1-CGFP-HA-His Vector for Mammalian Cell-Free Protein Expression
(Ex-work price)
$ On demand
$ On demand
On demand
![](https://laboshop.ae/public/storage/files/rKwkPquNCWRNoCaSjApQwAIPvHKmAaQzoUzAJVWQ.png)
Invitrogen™ pENTR™/D-TOPO™ Cloning Kit, with One Shot™ Mach1™-T1R Chemically Competent E. coli
(Ex-work price)
$ On demand
$ On demand
On demand
![](https://laboshop.ae/public/storage/files/ZulR3V3Knx6do2PUVwjaFM72Q8Yx49UwE738LePl.jpg)
Invitrogen™ Baculovirus Expression System with Gateway™ Technology
(Ex-work price)
$ On demand
$ On demand
On demand
![](https://laboshop.ae/public/storage/products/September2021/tdJNsQbZ1jakE7xlYejK.jpg)
Invitrogen™ pShooter™ Mammalian Expression Vector (pCMV/myc/nuc)
(Ex-work price)
$ On demand
$ On demand