Invitrogen™ pBAD/gIII Kit
On demand
Brand origin :
United States
Expiry time :
On demand
Delivery time :
1 Week/s
Delivery cost :
On demand
Unit size :
1 Kit
Order number :
V45001
Invitrogen™ pBAD/gIII Kit
The pBAD/gIII Kit provides all of the necessary reagents to express recombinant proteins under the tight regulation of the araBAD promoter. To simplify purification, the vector pBAD/gIII encodes a leader peptide which directs the recombinant protein into the
periplasmic space.
A set of three vectors is provided (A, B, and C). Each has the C-terminal tag in a different reading frame relative to the multiple cloning site to simplify in-frame cloning of your gene.
Features
- The araBAD promoter for tightly regulated expression
- Translation initiation signals for optimized E. coliexpression
- Leader peptide from the bacteriophage fd gene III protein (gIII) for secreted expression
- C-terminal polyhistidine (6xHis) tag for purification with nickel-chelating resin
- C-terminal c-myc epitope for detection and analysis with an Anti-myc Antibody
Contents
- The pBAD/gIII Kit includes 20 µg each of pBAD/gIII A, B, & C, 20 µg of pBAD/gIII/calmodulin, 1 ml 20% L-arabinose, LMG194 and TOP10 E. coli stabs.
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