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Invitrogen™ pBAD/gIII Kit

Out of stock
Brand origin : United States
Expiry time : On demand
Delivery time : 1 Week/s
Delivery cost : On demand
Unit size : 1 Kit
Order number : V45001
$ On demand

Invitrogen™ pBAD/gIII Kit

 

The pBAD/gIII Kit provides all of the necessary reagents to express recombinant proteins under the tight regulation of the araBAD promoter. To simplify purification, the vector pBAD/gIII encodes a leader peptide which directs the recombinant protein into the

periplasmic space. 

A set of three vectors is provided (A, B, and C). Each has the C-terminal tag in a different reading frame relative to the multiple cloning site to simplify in-frame cloning of your gene.

 

Features

  • The araBAD promoter for tightly regulated expression
  • Translation initiation signals for optimized E. coliexpression
  • Leader peptide from the bacteriophage fd gene III protein (gIII) for secreted expression
  • C-terminal polyhistidine (6xHis) tag for purification with nickel-chelating resin
  • C-terminal c-myc epitope for detection and analysis with an Anti-myc Antibody

 

Contents

  • The pBAD/gIII Kit includes 20 µg each of pBAD/gIII A, B, & C, 20 µg of pBAD/gIII/calmodulin, 1 ml 20% L-arabinose, LMG194 and TOP10 E. coli stabs.
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https://www.thermofisher.com/order/catalog/product/V45001?SID=srch-srp-V45001#/V45001?SID=srch-srp-V45001

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