Thermo Scientific™ pTK-Cypridina Luc Vector for Luciferase Assays
On demand
Order number :
16151
Thermo Scientific™ pTK-Cypridina Luc Vector for Luciferase Assays
The Thermo Scientific pTK-Cypridina Luc Vector is a multiple cloning site plasmid designed to accept a promoter sequence for study of gene regulation using the naturally secreting Cypridina luciferase reporter.
The Cypridina Luc Vectors contain a gene cloned from the marine ostracod, Cypridina noctiluca. The gene encodes a naturally-secreted bioluminescent Cypridina luciferase (62kDa), which enables measurement of the reporter activity in media (for real-time assays) and in cell lysates. The pMCS vector contains a multiple cloning site for cloning a promoter to study its regulatory potential.
Features
- Naturally-secreting Cypridina luciferase gene, optimized for high expression in mammalian systems
- Multiple cloning site (MCS) provides versatility for transfer of regulatory elements from one plasmid to another
- Transcription termination site (Ter), Lac operator (Lac O1), and transcriptional pause site (TPS) used to minimize background by reducing transcriptional read-through
- Both puromycin (Pur) and ampicillin (Amp) markers for drug selection in mammalian and bacterial cells, respectively
- High-copy pUC bacterial DNA replication origin
- Two control vectors available with strong (CMV) or weak (TK) constitutive promoters for co-transfection and normalization
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